This book presents a collection of high-quality research papers, presented at the Second International Symposium on Bioinformatics and Biomedicine (BioInfoMed'2022). It offers a comprehensive look into some of the fastest growing fields of science, such as biomedicine, bioinformatics, artificial intelligence, and mathematical modeling. The different chapters of the work include both practical solutions and strictly scientific considerations expanding knowledge about the future bioinformatics and biomedical engineering challenges. We believe that the presented works will have a great impact not only on the development and the application of new methods for modeling, decision making and data mining in healthcare and biomedicine, but also it will provide a source of inspiration for researchers who can implement the proposed methods into their practice and scientific studies.